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vector sh nc tr20003  (OriGene)


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    Structured Review

    OriGene vector sh nc tr20003
    PLCε regulates EXO1-mediated cell cycle, proliferation and apoptosis through nuclear translocation of p-ATM. A. The BCa cells were infected with vector-shEXO1, western blotting detected the expression of EXO1. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9, and BAX <t>in</t> <t>sh-NC</t> and sh-EXO1 groups. C. The BCa cells were infected with vector-EXO1, western blotting detected the expression of EXO1. D. Western blotting analysis detected the expression of PLCε, EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX in sh-EXO1 groups or/and Ad-shPLCε groups. E. Detection of p-ATM expression in nuclear and cytoplasm by western blot. F. Western blotting analysis showing that Ad-shPLCε reduced EXO1 level with 13 nM KU-55933 for 48 h. G. Immunofluorescence staining analysis demonstrating that Ad-shPLCε disrupted p-ATM nuclear translocation stimulated by cisplatin. Magnification: 400×. Bars = 100 μm. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.
    Vector Sh Nc Tr20003, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector sh nc tr20003/product/OriGene
    Average 95 stars, based on 256 article reviews
    vector sh nc tr20003 - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1"

    Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

    Journal: American Journal of Cancer Research

    doi:

    PLCε regulates EXO1-mediated cell cycle, proliferation and apoptosis through nuclear translocation of p-ATM. A. The BCa cells were infected with vector-shEXO1, western blotting detected the expression of EXO1. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9, and BAX in sh-NC and sh-EXO1 groups. C. The BCa cells were infected with vector-EXO1, western blotting detected the expression of EXO1. D. Western blotting analysis detected the expression of PLCε, EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX in sh-EXO1 groups or/and Ad-shPLCε groups. E. Detection of p-ATM expression in nuclear and cytoplasm by western blot. F. Western blotting analysis showing that Ad-shPLCε reduced EXO1 level with 13 nM KU-55933 for 48 h. G. Immunofluorescence staining analysis demonstrating that Ad-shPLCε disrupted p-ATM nuclear translocation stimulated by cisplatin. Magnification: 400×. Bars = 100 μm. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.
    Figure Legend Snippet: PLCε regulates EXO1-mediated cell cycle, proliferation and apoptosis through nuclear translocation of p-ATM. A. The BCa cells were infected with vector-shEXO1, western blotting detected the expression of EXO1. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9, and BAX in sh-NC and sh-EXO1 groups. C. The BCa cells were infected with vector-EXO1, western blotting detected the expression of EXO1. D. Western blotting analysis detected the expression of PLCε, EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX in sh-EXO1 groups or/and Ad-shPLCε groups. E. Detection of p-ATM expression in nuclear and cytoplasm by western blot. F. Western blotting analysis showing that Ad-shPLCε reduced EXO1 level with 13 nM KU-55933 for 48 h. G. Immunofluorescence staining analysis demonstrating that Ad-shPLCε disrupted p-ATM nuclear translocation stimulated by cisplatin. Magnification: 400×. Bars = 100 μm. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

    Techniques Used: Translocation Assay, Infection, Plasmid Preparation, Western Blot, Expressing, Immunofluorescence, Staining



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    vector sh nc tr20003  (OriGene)
    95
    OriGene vector sh nc tr20003
    PLCε regulates EXO1-mediated cell cycle, proliferation and apoptosis through nuclear translocation of p-ATM. A. The BCa cells were infected with vector-shEXO1, western blotting detected the expression of EXO1. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9, and BAX <t>in</t> <t>sh-NC</t> and sh-EXO1 groups. C. The BCa cells were infected with vector-EXO1, western blotting detected the expression of EXO1. D. Western blotting analysis detected the expression of PLCε, EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX in sh-EXO1 groups or/and Ad-shPLCε groups. E. Detection of p-ATM expression in nuclear and cytoplasm by western blot. F. Western blotting analysis showing that Ad-shPLCε reduced EXO1 level with 13 nM KU-55933 for 48 h. G. Immunofluorescence staining analysis demonstrating that Ad-shPLCε disrupted p-ATM nuclear translocation stimulated by cisplatin. Magnification: 400×. Bars = 100 μm. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.
    Vector Sh Nc Tr20003, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector sh nc tr20003/product/OriGene
    Average 95 stars, based on 1 article reviews
    vector sh nc tr20003 - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    PLCε regulates EXO1-mediated cell cycle, proliferation and apoptosis through nuclear translocation of p-ATM. A. The BCa cells were infected with vector-shEXO1, western blotting detected the expression of EXO1. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9, and BAX in sh-NC and sh-EXO1 groups. C. The BCa cells were infected with vector-EXO1, western blotting detected the expression of EXO1. D. Western blotting analysis detected the expression of PLCε, EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX in sh-EXO1 groups or/and Ad-shPLCε groups. E. Detection of p-ATM expression in nuclear and cytoplasm by western blot. F. Western blotting analysis showing that Ad-shPLCε reduced EXO1 level with 13 nM KU-55933 for 48 h. G. Immunofluorescence staining analysis demonstrating that Ad-shPLCε disrupted p-ATM nuclear translocation stimulated by cisplatin. Magnification: 400×. Bars = 100 μm. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

    Journal: American Journal of Cancer Research

    Article Title: Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1

    doi:

    Figure Lengend Snippet: PLCε regulates EXO1-mediated cell cycle, proliferation and apoptosis through nuclear translocation of p-ATM. A. The BCa cells were infected with vector-shEXO1, western blotting detected the expression of EXO1. B. Western blotting analysis detected the expression of EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9, and BAX in sh-NC and sh-EXO1 groups. C. The BCa cells were infected with vector-EXO1, western blotting detected the expression of EXO1. D. Western blotting analysis detected the expression of PLCε, EXO1, cyclinD1, c-Myc, PCNA, BCL2, caspase 3, caspase 9 and BAX in sh-EXO1 groups or/and Ad-shPLCε groups. E. Detection of p-ATM expression in nuclear and cytoplasm by western blot. F. Western blotting analysis showing that Ad-shPLCε reduced EXO1 level with 13 nM KU-55933 for 48 h. G. Immunofluorescence staining analysis demonstrating that Ad-shPLCε disrupted p-ATM nuclear translocation stimulated by cisplatin. Magnification: 400×. Bars = 100 μm. *P ≤ 0.05, **P ≤ 0.01, and ***P < 0.001.

    Article Snippet: EXO1 human tagged ORF clone vector (RC200547), pCMV6-Entry vevtor (PS10001), vector-sh-EXO1#1 (TR304725) and vector-sh-NC (TR20003) were purchased from OriGene Technologies.

    Techniques: Translocation Assay, Infection, Plasmid Preparation, Western Blot, Expressing, Immunofluorescence, Staining